Construction Of Plant Transformation Vector And Transferring Into Potato Of The Plrv 56kd Protein Gene

The 56KD protein gene & 3?part non encoded region of the PLRV Chinese isolates which contained in plasmid pLR56 were constructed into binary vector plasmid pROKII, which was introduced into the commercial potato cultivars Dongnong?03 by the agrobacterium tumefaciens梞ediated method. The optimized media systens of plant regeneration for leaf and stem organs were summarized @MS+2.25mg/L 6桞A+4mg/L NAA?Weeks桵S+2.25mg/L 6桞A+2mg/L ZT+5mg/L GA3 ㎝S+2. 25mg/L 6桞A+4mg/L NAA?Weeks ?MS+O. 3 GA3 respectively. The optimum transformation system for explant and regenerative shoots?rooting is that the concentration od kanamycin and acetosyringone were 5Otng/L and OD~00O. 2 respecting, and the cocultivation time is 2 days after 2 days pre梒ultivation.