| The regeneration systems in vitro had been systematically established in You Nane (Prunus salicina Lindl cv. Younai) and its genetic transformation was preliminarily studied. As to regeneration systems, an optimal regeneration system was established by shoot tip culture; another good regeneration system which the test-tube plantlet stem segments could regenerate adventitious buds directly, had also been established; moreover, adventitious buds regenerated from leaf of test-tube plantlet, cotyledon and epicotyl had also been studied. As to genetic transformation, using the regeneration system of adventitious buds from stem segments as the transformation recipient system, the hairy root had been induced by Agrobacterium rihizo genes mediated and ACO gene had been integrated into genome by Agrobacterium tumefaciens mediated. The main results were as follows:1. Asexual regeneration system of shoot tip cultureStem segments with axillary buds were sterilized in 1 g ?U?HgC12 for 10mm. Survival percentage of explants was 56.7% after 20d of culture. The most optimal induction medium was MS+2.Omg·L-1Ze+30mg·L-1Ad+0.2mg·L-1IAA, in which the differentiation rate of explants could be up to 66.4%. The most optimal proliferation medium was MS+4.Omg·L-16-BA+0.2mg·L-1 IAA, the proliferation coefficient of buds being 4.5. The most optimal growth medium for buds was MS+1 .0mg·L-1Ze +30mg·L-1Ad+0.2mg·L-1IAA+400mg·L-1LH, in which single bud could grow average 1.5cm in one generation. The most optimal rooting medium was l/2MS +0.2mg·L-1IBA+l.Omg·L-1IAA, rooting rate being up to 100%. Clinical powder 200mg·L-1Cef+200mg·L-1Cb added to the proliferate medium could wholly resolve the endogenous bacteria produced in subculture.2. Regeneration system of adventitious buds from stem segmentsHigh concentration of cytokinin was needed to stem segments induced into adventitious buds. The optimal inducible medium were MS+4.Omg·L-1Ze+4.Omg. ·L-16-BA+l.Omg·L-1IAA+400mg·L-1LH, in which the adventitious buds regeneration frequency and average regeneration buds of basal part, middle part and upper part of stem could achieve 100%, 6.50, 83.3%, 4.25 and 62.5%, 3.80 respectively. During initiation culture stage, dark culture and 300-400mg U?LH were advantage to regeneration of adventitious buds. In addition, adventitious budsregeneration frequency of basal part of stem after longitudinal cutting was up to100%, average regeneration buds were 4.25.3. Regeneration system of test-tube plantlet leaf, cotyledon and epicotylInduction rate of test-tube plantlet leaves regenerated into adventitious buds was low, the most optimal result in this study was 13.3%, which was attained in the medium MS+8.Omg U?-BA+l.Omg U扞AA, but their average regeneration buds were up to 4.0.The regeneration of adventitious buds from cotyledon and epicotyl were different based on various treatments. The optimal inducible medium of cotyledon and epicotyl was MS+10.Omg . U?-BA+l.Omg U?IAA. Adventitious buds regeneration frequency and average regeneration buds of cotyledon after longitudinal cutting (surface cutting) and horizontal cutting (near cotyledon petiole and surface cutting) were 60.0%, 3.21 and 63.3%, 3.02 respectively. Adventitious buds regeneration frequency and average regeneration buds of epicotyl after horizontal cutting and longitudinal cutting (surface cutting downward) were 76.3%, 1.52 and 52.7%, 3.14 respectively.4. Hairy root-induction via Agrobacterium rhizogenes-mediatedVia Agrovacerium rhizogenes strains M and RI 601 mediated, the hairy root induction potential in stem segment was stronger than the strains A and Ri 000. As to the transformation recipient, basal part of stem was prior to middle part of stem, upper part of stem or whole plant. Basal parts of stems were invaded by strains M and R1601 With the method of direct-inoculation. As the stem segments inserted medium upward, the hairy root induction rates and average hairy roots were 57.4%, 3.29 and 58.3%...
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