Research On The Proliferation And Differentiation Of Rat Preadipocytes

The In vitrO models of adipocyte differentiation were used to inveStigate theproliferation and differention of primary preadipocyte from rat adipose tissue in serumsuPPlemented.Preadipocytes were seeded in plating medium containing FBS--free(fetal bovineserum), l%FBS, l0%FBS and 20%FBS. PreadiPOcytes were seeded in diferent mediaincluding Ml99 medium, RPMIl640 medium and DMEM:HamF-l2(1:l) medium.Preadipocytes from adipose tissue of 20-d-old rat, 40-d-old rat and 60-d-old rat wereseeded in plating medium containing l0%FBS. Aftetr seeding by l6 hours, the numbersof preadipocytes dshed tO the platC were coamed by using a coulter counter. The rate ofadhension(RA) of l%FBS, l0%FBS and 20%FBS was higher than the RA ofFBS-free(P<0.0l). the RA of l0%FBS and 20%FBS was higher than the RA ofl%FBS(P<0.0l). the RA of l0%FBS was not different from the RA of 20%FBS. Theresults show that FBS have an significan effect on the attachInent of preadipocytes fromrat adipose tissue. the RAs of Ml99 medium, RPMIl640 medium andDMEM:Ham-Flz(l:l) medium were not different from each other. The attachInent ofpreadipocytes showed no dependence on media. The ax ofpreadipocytes from the adiposetissue of 20-d-old rat was more higher than that of 60-d-old rat(P<0.0l). the attchment ofthe preadipocytes from adipose tissue of young rat was more easily than that of old rat.FBS was added to the growh medium at concentration of l% FBS, 5%and l0%FBS. The proliferation of the preadipocytes of 5% FBS and l0% FBS were higher thanthat of l%FBS(P<0.0l). The proliferation of the preadipocytes of l0% FBS were higherthan that of 5%FBS(P<0.05). The exPeriment indicated that FBS have a highly signifinaneffect on the proliferation ofpreadipocytes. In order to describe the proliferating pattem ofpreadipocytes, the growth curve of the preadipocytes from adipose tissue of 20-d-old ratwas determined by the method of cell counting in l0%FBS supplemented. The...