Effects Of Fluid Shear Stress On Expression Of Proto-oncogenes C-fos And C-myc In Cultured Human Umbilical Vein Endothelial Cells

Vascular endothelial cells (ECs) line in the inner surface of blood vessels and contact with blood flow directly, so they are continually exposed to the hemodynamic stress that are generated when blood flows through the vasculature. There are many physiological functions in ECs. The alteration of ECs in structure and function can affect blood fluidity, vascular strain and the regulation of penetration, even cause some diseases. The shear stress (SS) generated by blood flow is just one of the important factors that affect modality and function of ECs. Proto-oncogenes are induced by some signal transduction pathways after ECs being stimulated. Moreover, more and more attention is paid to the proto- oncogenes in recent years. To reveal effects of SSes on expression of proto-oncogenes, we chose cultured human umbilical vein endothelial cells (HUVECs) as object of our study used the parallel plate flow chamber to controll the value and duration of SS and then dealt cultured human umbilical vein endothelial cells (HUVECs) with immunocytochemistry method and image analysis software in order to disclose the expression rule of proto- oncogenes c-fos and c-myc caused by different SSes. It will be very instructive to farther explore ECs signal transduction pathways and reveal pathogenesis of some diseases such as tumor. We found that proto-oncogene c-fos protein level in stationary (no effect of SS) was very low. But c-fos protein level rapidly increased after we imposed SS on ECs. In our experiment, the value of SS used was 4 dynes/cm2 and 10 dynes/cm2. Under the 4 dynes/cm2 SS, c-fos protein level added along with time. The level reached the peak at 1.0 h and there was very significant difference compared with the control group (p