| It is becoming more and more difficult to discover new pesticides, due largely to the increasing environmental and highly competitive nature of the agrochemical market. In an effort to maximize the chances of discovering new active compounds, the emphasis in recent years has been on increasing the number of compounds tested, both on the primary screens and on the follow-up screens. The objective of this research is to establish a new herbicidal screening method which quick, accurate and simple.In this research, we tested 43 herbicides which include 20 different chemical classes and 11 different modes of action using 5 unicellular green algae. The results showed that Chlorella pyrenoidosa was more sensitive than the other 4 algae and was suitable as target organism during herbicide screening. This method of herbicidal screening provides many advantages in such as speed, simplicity, cost, sensitivity and so on. It just compensates for the inadequacy of conventional screens such as Petri dishes. Therefore, the in-vitro systems can form a second screening net to ensure that potential compounds not be missed. Morever, the results of microscreening also showed this method is suitable to make the requirements for high-throughput screening(HTS) in vitro.Besides, we have studied the effects of several frequently used solvents and adjuvants on the growth of Chlorella pyrenoidosa and determined the solvent system which can be used to solve Technical Compounds(TC). The environmental conditions were also determined in this test.The establishment of this method of herbicidal screening using Chlorella pyrenoidosa as model organism will be helpful to the development of herbicide pharmaceutial in China. And what's more, it is possible to screen insecticides, fungicides, and algicides using this method.
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