| In order to develop an accurate and efficient method for the identification of tiger DNA,mtDNA Ctyb sequences of 5 tiger subspecies and 6 other feline species and 6 cervid species were downloaded from Genbank,and compared by Wdnasis(V2.5) software. Considering the essential doctrine of designing PCR primers,two regions in which all tigers share an unique hyplotype against other species were selected to design a pair of primers (primer 1,2) to amplify a tiger cytb fragment specifically. PCR tests were performed with DNAs extracted from muscle,visceral tissues,skins or hairs of Siberian tigers,South-China tigers and 8 other feline species and 6 non-feline species using this primer set. The results indicated that the primers are specific to tiger DNA and any other species DNA can't be amplified in PCR. Identification of tiger DNA by this means achieved accurate,sensitive,reproducible and reliable.Compared with the presented reports,the method of mtDNA isolation from hair shaft established in this paper breaks the restrictions to the hair freshness and make the shed hair without follicle and kept for many years valuable in identification. This paper provides a powerful tool to identify tigers by using tiger products and field samples.
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