Study Of Effect Of AVP And OT On Membrane Of DRG Neurons And Its Mechanisms In Rats

ObjectiveTo study the effect of arginine vasopressin (AW) and oxytocin (OT) on neurons of dorsal root ganglion (DRG) and its mechanisms in the rat.Method1. Intracellular recording technique was used in the experiments.2. X+SE and t test were used to statistically analyze the experimental data.Results1. AW or OT induced hyperpolarization in most of the DRG neurons.2. The membrane conductance of the DRG neurons was increased 19.32% by AW and 16.26% by OT, respectively.3. Supervision with Na+-free or Cd2+ (b10cker of Ca2+ channel) failed to affect the AVP- and OT-induced membrane hyperpolarization of the DRG neurons. Superfusion with BSS containing tatraethylammonium iodide (TEA, b10cker of K~ channel), however, reduced obviously the AW-and OT-induced hyperpolarization of the neurons.4. The AW- and OT-induced hyperpolarization of the neurons could beb10cked by antagonist of AVP Vi and OT receptor, respectively.Conclusion1. AVP V1 and OT receptors exist on the DRG neuron membrane.2. AVP- or OT-induced hyperpolarization of membrane potential is caused by outf10w of K+ mediated by AVP or OT receptors on the membrane of the neurons.3. AVP and OT might be involved in modulation of sensory input information through inhibiting the DRG neurons.