Proteomics On Aeromonas Hydrophila

The human genome project will be completed in advance. and functionalgenomic era will be coming. Proteomics will be not only the major task in lifesciences in the 21st century,but also far reaching implications in innovations formedicine, agriculture and industrial production.Proteomics on Aeromonas hydrophila, one of the important pathogens inaquaculture and human, has been characterized in this study The techniques usedincluded two-dimensional electrophoresis (2-DE) and mass spectrometer (MS).Proteins were identified with peptide mass fingerprinting (PMF).Firstly, the first molecular anatomic profile of Aeromonas hydrophila wasestablished, 40 proteins were identitied from analysis of the PMF of l24 proteins. Ofthe 40 proteins, 17 were from the source of Aeromons hydrophila genes/proteins. Themolecular anatomization will provide useful data to further studies at the level ofproteomics.Then. we analyzed the immunogenic proteins of Aeromonas hydrophila, andidentified 5 immunogenic proteins by the use of 2-DE and Western-blot. The fiveproteins were suggested to be Flagellin FlgB, hypothetical protein,exopolyphosphatase, groEL, EF-G2. Of 5 immunogens. groEL and EF were reportedin other pathogens. The further study on theses conserved antigen will be helpful todevelop new vaccines.At last, the profiles of different generations of Aeromonas hamphila and itsresistance to antibiotics were detennined at the level of sub-proteomics. The results ofboth of virulent attack test and 2-DE were paralleled in the following generations,with decrease of virulence of Aeromonas hamphila in the former, and deletion oftoxin-related genes in latter. In the experiment of antibiotic resistance, the resultsshowed that 3 kinds of mechanisms of resistance to antibiotics might be involved. 1.Aeromonas hydrophila may pump the antibiotics through transporter protein.2.Aeromonas hydrophila recognizes the signal from the outside environment throughsensors, and suits for it. 3. Some transcriptional factors may be expressed to regulatethe expression of other genes to resist to antibiotics.These researches above will be importan to prevent and cure the diseases causedby Aeromonas hydrophila.