Studies On The Tissue Culture Of Pinellia Pedatisecta And Actinidia Deliciosa (Qinmei)

The tissue culture and plant generation were studied in the present paper by using hypocotyls and cotyledons of Pinellia pedatisecta and the leaves and petioles of Actinidia deliciosa(Qinmei). 1. Studies on the tissue culture of Pinellia pedatisectaThe hypocotyls, cotyledons of Pinellia pedatisecta were used as explants for tissue culture and the results showed:(1) The hypocotyls were the better explants for propagation of Pinellia pedatisecta. The explants of hypocotyls could produce calli when cultured on MS medium supplemented with BA 0.5 mg/L, NAA 0.5 mg/L for 7 days and then the calli could differentiate into adventitious buds later. The differentiation rate of adventitious buds could be up to 100 % after 25 days, and there were about 4.2 adventitious buds produced from each hypocoryl. When the adventitious buds grew to 2-3 cm in height, they were excised and inoculated on 1/2 MS medium supplemented with NAA 0.2 mg/L. After cultured for two weeks, the plantlets rooted. They were transferred into the substrate (vermiculiterhumus = 1:1),and the survival rate of the regenerated plantlets was 72 %. The studies also showed that, BA had the better effects on the promoting of the adventitious buds differentiation of the hypocotyls than KT and ZT. 1/2 MS medium and darkness benefited the rooting of Pinellia pedatisecta in the process of tissue culture.(2) All the explants could produce calli in 25 days after they were cultured on differentiation medium supplemented with MS+ 2,4-D 1.0 mg/L+ IBA 0.5 mg/L. The effects of hormones on the callus formation were also studied in this paper. The studies of the differentiation of adventitious buds are undergoing.2.Studies on the tissue culture of Actinidia deliciosa(Qinmei)The leaves and the petioles of Actinidia deliciosa(Qinmei) were used as explants and the result showed:(1) Calli were produced and further differentiated when the leaves had been cultured on MS medium supplemented with ZT 1.0 mg/L, NAA 0.5 mg/L for 20 days. The differentiation rate was 100 % and there was about 3.54 adventitious buds producedper explant. For the induction rate of adventitious buds of the leaves, the effect of ZT was the best, BA followed, but KT was not obvious. The leaves could produce light-yellow or green calli when cultured on MS medium supplemented with BA ( 0.5 -4.0mg/L) and 2,4-D(0.5-4.0 mg/L). The calli produced adventitious buds after they had been subcultured on MS medium supplemented with ZT 0.5 mg/L and NAA 0.5mg/L for 2-3 times culture. The higher ratio of BA and 2,4-D benefited the formation of the buds and the lower ratio of BA and 2,4-D benefited the formation of the calli in the process of leaf callus induction.(2) All the'petioles produced the calli when cultured on MS medium supplemented with BA 1.0 mg/L and IBA 0.5 mg/L after 10 days. When the calli were subcultured on this medium for 20 days, the adventitious buds were differentiated and the rate was 37.5 %. About 1.76 adventitious buds were produced per petiole. The effect of BA on the regeneration of adventitious buds was better than KT and ZT. The combination of BA and IAA, IBA or NAA could significantly improve the regeneration rate of the petioles.The studies on the tissue culture and the plant regeneration of Pinellia pedatisecta and Actinidia deliciosa(Qinmei) supply not only the bases for the improvement of breed, acceleration of breeding and rapid propagation of Pinellia pedatisecta and Actinidia deliciosa(Qinmei).established, but also a new experimental system for many foundational theory study, such as physiologic development, cell differentiation and morphogenesis etc.