Screening For A Endurable High Temperature And High Productive Mutant And The Study Of Its Productive Condtion

Ethanol is an important production and play an important role in the whole economy .It could be widely applied in the society .There are two methods to product ethanol :one is the fermentation by the microbe,the other is synthesized by the chemistry .The first way is principal in our country .The fermentative strain is yeast generally and it can just product ethanol on the condition of 29-35 .The temperature on the later stage of fermentation reaches 38 .In order to sustain the production ,the factory have to spend large of energy and cold water. The program of breeding the strain of yeast which can endure high temperature and have high productivity is carrying on all around the world .The paper adopted the common breeding way by series of mutagen treating to the yeast's cell and spheroplast.Finally,the excellent mutant 75L 3555 was obtained.Its nature of the production was determined and it can product ethanol on the condition of 40 and have high fermentative speed and stable production.On the other hand, the test was made that the best fermentative condition was found.In the malt juice,adding 0.02%K+ or 0.03% A13+ can increase the production strikingly and adding some degree concentration trahalose can increase the production too and cut short the time of taming.In the study of using molasses producting ethanol,we can obtain high productivity contained the following composition:Nitrogen source 1.5% maize and 0.075%(NH4)2SO4. Phosphorus source 0.2%KH2PO4. Molasses concentration 22X, pH4.5.The mutant (75L3566) can compensate the shortcoming of the productive strains in our country which can endure high temperature and product normally. The result of the paper can't find in data in our country presently.