Studies On Tobacco Cell Culture And Kinetics Of CoQ₁₀ Production

Quick-growth cell lines were obtained after callus culture and suspension culture, starting with sterile seedling of Yellow Bright, a breed of Nicotiana tabacum with high CoQ10 content. The optimal conditions for the growth of tobacco cell is MS + 6-BA 0.2 mg/L + NAA 2.0 mg/L, combined with 3% sucrose and 8% original cell content, which made the maximal dry weight of cell product to be 36.7 g/L. Several constituents affecting tobacco cell growth and the production of CoQio were studied. The optimal temperature for cell growth and product formation 25 . As for growth regulators, 0.5 mg/L IAA has been shown to increase effectively the content of CoQ10, though it did not promote the growth of tobacco cell. Yield of CoQ10 was increased by 54.6% to around 9.34 mg/L. The adding of some nutrients could considerably promote the formation and accumulation of CoQ10. For example, HCL-thiamine at 4.0 mg/L enhanced the content of CoQ10 by 65.2% to be 9.96 mg/L, 100 mg/L L-Cysteine made it to be 8.99 mg/L. With precursor feeding, the content of CoQio could be 9.35 mg/L at 2.0mg/L L-Phenylalanine, and 9.92 mg/L at 3.0 mg/L P-Hydroxybenzoid. Based on the experiment, we established a kinetic model to characterize the growth of tobacco cell growth, limiting substance consumption and CoQ10 production. This model can preferably fit the experimental data with a high relative modulus above 99%.