Studies On Intracytoplasmic Sperm Injection (ICSI) In Giant Panda Spermatozoa (Ailuropoda Melanoleuca) And Golden Hamster Oocyte

In this paper, we studied the effects of different hormone dosage on superovulation of golden hamsters; In the same time, we also investigated the factors influencing fertilization ability including the mechanical damage, die capacitation of sperm and electric stimulation of ova by injecting such sperms of the giant panda as normal sperm, sperm head and dead sperm into the hamster ova respectively using the micromanipulator.The results obtained were as follows: (1) The golden hamsters were treated with three different hormone dosage to make a superovulation. The average of ova recovered with 30IU PMSG+30IU HCG and 40IU PMSG + 40IU HCG treatment was not significant. But the average of ova was significantly higher with 30IU PMSG+30IU HCG and 40IU PMSG + 40IU HCG treatment than that with 50IU PMSG + 50IU HCG treatment (31.57, 32.83 vs.25.60; P<0.01). There was no significant difference in the number of ova used (98.44% vs.98.18%, PX).05) between the two hormone combinations 30IU PMSG + 30IU HCG and 40IU PMSG + 40IU HCG. The ova used of the two groups was significantly higher than the group 50IU PMSG and 50IU HCG(98.44%, 98.18% vs.94.67%; P<0.01). (2) Three different conditions sperms were injected into the ova of golden hamsters respectively. After injection, the rate of viability was not significant (84.15% vs.81.72% vs. 82.81%; PX).05). The rate of fertilization of normal sperms, sperm heads and head sperms was 26.09%, 11.84% and 13.21% respectively. The rate of fertilization of normal sperms was significantly higher than that of sperm heads and dead sperms (P<0.01). The rate of fertilization between sperm heads and dead sperms was not significant (P>0.05). (3) The sperms capacitated in vitro were injected into the ova, and the rate of viability was not significant(85.37% vs.87.18%? 82.50% vs.81.25%; 84.31% vs.82.46%; P>0.05). The rate of fertilization was a little high, but the difference was not significant (28.57 % vs. 29.41 %; 12.12 % vs. 12.82 %; 13.95 % vs. 14.89%; P>0.05). (4) The ova were treated with electric stimulation before injection, theviability of the ova was not significant after injection (79.37% vs.83.80%; 78.38% vs.80.28%! 78.87% vs.77.33%; P>0.05). The rate of fertilization of normal sperms was a little high, but the difference was not significant (28.00% vs.29.63%? P>0.05). The rate of fertilization of sperm heads and dead sperms was significantly increased by the electric simulation treatment of ova (13.79% vs.19.30%; 12.50% vs.18.97%; P<0.01).Hybrid embryos could not develop further.The results above showed: (1) The number of ova collected was much more with the 40IU PMSG and 40IU HCG dosage combinations. (2) The damage sperms of the giant panda had the ability of fertilization. (3) The capacitation in vitro of sperms could increase the rate of fertilization on ova, but the difference was not significant. (4) Appropriate electric stimulation could significantly increased the rate of fertilization of giant panda's damaged sperms. The rate of fertilization of normal sperms was a little high, but the difference was not significant...