The Studies On The Difference Of Catalytic Activity Between DNA And POD

This thesis is made of two parts: the reports and the review. In the first part, the research is mainly reported about the difference of catalytic activity between DNA and POD. To compare the difference, the author selected several kind of substrates which were treated with different ways and some differences were found. The first one was substrates catalyticed by DNA or POD had different absortion peak . such as when the substrates was benzidine and a-naphthol,the absortion peak was 450nm after catalyticed by DNA, and 501nm after catalyticed by POD ,if changing substrate to benzidine and pyrocatechol, the absortion peak was 503nm after catalyticed by DNA and 603nm after catalyticed by POD. The second difference was the different result after HPLC: The result of HPLC (substrates: benzidine and a-naphthol) showed that the products under the affect of DNA and POD had different value on the peak and the area. At the same time ,on the condition of different value of pH (substrates: benzidine and a-naphthol), catalytic reaction reveal distinct results as well. The last, to the other seven kinds of substrates, DNA didn't have any catalytic activity. On the contrast, POD had apparent activity to these substrates. The studies showed that DNA has the peroxidase-like activity , but this activity is unlike POD which is protein. On the second part, the history of research on deoxyribozyme was reviewed and the theory and practice value of deoxyribozyme were discussed.