Study On Micronucleus Frequency And Heat Shock Induced By Environmental Factors In Plant

Following the progress of science and technology, the development of industry and the improvement of standard of people' s living, the problems of environment prick up increasingly. The imperils can turn to three kinds: aberration, carcinogen and mutant. And the root of them rest with mutant, because both aberration and carcinogen often result from mutant. Thus, monitoring all kinds of mutant factors and materials in our environment is always the study hotspot by many biologists. Vicia micronucleus technology is just one of rather efficient measures of monitoring mutagens. In the other hand, because of the negative effects on the organisms by the surroundings, they themselves have to adapt to live. So they have tried to acquire a series of adaption mechanism to avoid or partly alleviate the bad effects step by step during the long evolutional period. The HSPs belong to these sorts of proteins which can be induced by heat shock and help avoiding the hurt from it. This experiment chiefly study on the comparative effects of micronucleus frequency on Vicia root tips after treating with two familiar factors: heat shock and Cr6+. Then by use of barley, discuss preliminaryly the HSPs induced by temperature and fish out a suit of quite economical and convenient method of two-dimensional gel electrophoresis of soluble proteins from the leaves of plant.1. Effects on micronucleus frequency of Vicia root tips induced by environmental factorsApplying with micronucleus test of Vicia root tips, the paper comparatively study on the effects on micronucleus frequency of Vicia root tips after treating with heat shock and Cr6+, and confirm temperaturecan affect the cell nucleolus and chromosome of Vicia root tips as heavy metal. And the genetic toxicological effects and imperils are also comparable. When the treating temperatures increase by degrees from 28癈 to 34℃, the micronucleus frequency of Vicia root tips increase though it is very slight. Also, between 37℃-43℃, the micronucleus frequency of Vicia root tips increase clearly. But when the temperature add up to 46℃, the micronucleus frenquency decrease on the contrary. Such are also tallied with the relative experimental results of Cr6+ and other heavy metals ago.2. Research on the isolation and two-dimensional gel electrophoresis of soluble proteins from the leaves of plantBy use of Vicia, this paper studies on soluble proteins of leaves of plant, including isolation, the course of two-dimensional gel electrophoresis, and dying. Following these courses, stable and rather clear map of two-dimensional gel electrophoresis is obtained through silver dying, and more than 300 spots can be observed by naked-eye. Most of their isoelectric points are from 4.0 to 7. 0, and their molecular weight are from 14 to lOOkDa.3. The HSPs of the leaves from barley induced by temperature Applying with the protein marker of 35S-Met, this paper study on thetesting of pH values in the gel after IEF of two-dimensional gel electrophoresis. Linking to the map of two-dimensional gel electrophoresis, then analyze simply the relation between resolving power of two-dimensional gel electrophoresis and pH values in the gel after IEF. Moreover, this paper stresses on the differences of intensity of HSPs induced by heat shock in the dark and in the light. The results indicate, the pH values between 6 to 8. 4 in the gel are linear with the distances between the testing spots, and form the well-grads of pH values. So the effect of proteins separation and the resolving power are thebest of all. Besides, this experiment finds at first plants response more intensively in the dark than in the light at the same temperature of heat shock, and gets more synthetical HSPs. But the mechanism need furthermore study.