| Secondary database that play an important role in the research and development of biology is a vital research subject in the field of bioinformatics. At present, there are enormous 16S rDNA sequences of bacteria available in the Genbank. In this research all 16S rDNA sequences of bacteria are collected and the about two hundred species-specific oligonucleotide probes for various bacteria according to 16S rDNA have been designed and stored into database. And a user-friendly search system based on computer network has been constructed. This secondary database could help users to design oligonucleotide probes.There are a number of plant growth-promoting rhizobacteria in the rhizosphere of plants and it is meaningful for detecting, classifying and identifying these bacteria to research the interaction of plants and microorganisms and the efficiency of the microbial inoculums. In this research, familiar plant growth梡romoting rhizobacteria are selected to design respectively oligonucleotide probes which target for different classification levels (species, genera and family) of bacteria and will be fixed to specific slides and then relative bacteria are used to select specific probes which would be applied to detect unknown examples. These rhizobacteria include Azospirillum brasilense, Azospirillum lipoferum, Bacillus cereus , Bacillus megaterium , Bacillus pumilus , Bacillus sphaericus , Bacillus subtil is , Bacillus coagulans , paenibacillus polymyxa , Burkholderia gladioli , Agrobacterium , Alcaligenes , Arthrobacter, Bradyrhizobium, Burkholderia, Flavobacterium, Pseudomonas, Rhizobium, Streptomyces and Enterobacteriaceae. The results show that it is successful for most probes to produce positive signals of hybridization.
|
PDF Full Text Request