High Density Fermentation Of Ganoderma Lucidum And Monascus Purpureu And Development Research Of A New Medicine

Both Ganoderma lucidum and Monascus purpureus contain many bioactive substances which are healthful to humans. Lots of microbiologists and medical workers have paid much attention to these microorganisms and made some progress in primary studies. In order to increase the products yields from G lucidum and M. purpureus and improve their production level, these fungus were studied deeply in this paper.For the research of G lucidum, a series of experimnents were carried out to find out the optimum medium compositions and the optimum culitivation conditions. By using liquid fermentation method, batch and fed-batch were carried out. Finally, 9.29g/L of biomass of G lucidum was obtained and 2.208g/L of its polysaccharide was attain after 60 hour-fermentation. The synthesis rate of the polysaccharides was enhanced to 0.036g .h-1.L-1 which was 20 % higher than the data reported. In addition, we found that the synthesis method of G lucidum polysaccharides was unrelatable type with growth, and the polysaccharides was synthesized at the stable phase. According to the actual situation of the synthesis process, the yield of the polysaccharides would be improved if the stable phase had been prolonged by adjusting the metabolism artificially. These results indicate that the industrialized production of G lucidum polysaccharides by liquid fermentation is completely feasible.The downstream processing conditions of G lucidum polysaccharides and flavone were studied and the extracting technique routes of these were set up. With the G lucidum polysaccharides and flavone as the "monarch drug", Feng Shen Ling Capsules have been developed with radix astragali, radix salviae miltiorrhizae and radix scrophulariae as the "principal drug". The capsule has been proved effective in enhancing human immunity function and decreasing the blood sugar level.In the study of M. purpureus, the optimum conditions for its medium composition and culitivation were discussed. High density fermentation was carried out and the mycelium yield reached to 4.09g/L, which is about two times higher than the data of 2.18g/L reported in literature. However, the dynamic parameters of the Monacolin substances couldn't be investigated in the process of fermentation due to some reasons.In this paper, fermentation conditions of G. lucidum and M. purpureu were studied respectively in their dynamic parameters and metabolism regulation. This will provide important technological supports for further industrialized production. Furthermore, Feng Shen Ling Capsula developed from the fermentation products of G lucidum will provied a new promising medicine for curing diabetes.