The Cytology Of Inflorescence And The Molecular Mechanism Of Curd In Brassica

Brassica genus, as a complex species, contains many varieties with grossly different inflorescence phenotypes, including broccoli, cauliflower, cabbage, Chinese cabbage and so on. The difference results from the change of the development orientation of meristem in apex during ontogenetic process of these plants, so it is necessary to study these differencewith anatomical method. We compared with the cytologic variety of structure at main four development stages among Chinese cabbage, cauliflower and broccoli with paraffin method and with technique for scanning electron microscopy. And the cytoligic difference of apex structure during inflorescence development among the three variants of cole, such as cabbage, cauliflower and broccoli was also compared with. The result offers some basis for the study of the anatomical entity of different inflorescence in Brassica.Many explants are needed in plant transgene experiments. Previously, germ梖ree plants first be got from seeds, then the cotyledon, epicotyl and hypocotyls were cut as explants; or transgene was carried out when the plants flowered. In our experiment, meristem of the curd of cauliflower as explants, deleting the callus stage, were first directly differentiation buds, then buds were transformed to medium which can induce roots, at last regeneration plants were gained. And flowered plants were also quickly gained. This method can quickly offer enough materials for transgene in cauliflower.The early research on cauliflower mutant of arabidopsis thaliana and hometic genes in cauliflower suggests that either AP1 or CAL gene playsanimportant role in the curd formation. In order to study the molecular mechanism of curd, we adopt the widely used yeast two-hybrid system, CAL1 as a bait gene, to screen arabidopsis thaliana cDNA library, and found that a AMPK梐ctivated protein kinase bate2 subunit has an ability to interact with the CAL. The research on plant protein kinase(PK) suggests that PK has closely relationship with particular physiological response which is produced by environmental stress signals. In our tissue culture experiment of cauliflower , we also watched the weaken of the maintenance of the curd phenotype when natural growth condition was deleted. This suggests that AMPK perhaps has a indirectly role by CAL gene during the curd information of cauliflower.