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Study On The Inhibiting Effects Of Carnosine On Lipid Peroxidation In Vitro

Posted on:2005-03-21Degree:MasterType:Thesis
Country:ChinaCandidate:J H SuoFull Text:PDF
GTID:2120360125459293Subject:Animal Nutrition and Feed Science
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For the evaluation of antioxidant activity of Carnosine, the methods was used in the experiments, including the FRAP assay for carnosine of total antioxidative power , three Lipid Peroxidation systems (Yolk lipoprotein oxidation induced by Fe2+, LDL oxidation induced by Fe2+ and linoleic acid oxidation by Fe2+-VitC system), and liver homogenate auto-oxidation system.In partâ… , FRAP assay was employed to determine the total antioxidant power of carnosine .The results showed that total antioxidant power of carnosine was highest compared with beta-alanine, benzoate, dinethy sulfoxide.In part II ,Yolk lipoprotein oxidation induced by Fe2+and TBARS was measured as the oxidative index . The results illustrated that the inhibition rate (IR)(%) of 2.5, 5, 10, 20, 40mmol/Lcarnosine on TBARS formation of Yolk lipoprotein was 43, 52.6, 56.8, 71.1, 75.3,and the antioxidant effect of carnosine was higher compared with beta-alanyl,l-histidine, benzoate,dinethy sulfoxide. The activities of carnosine increased in a dose-dependent manner.In part III , LDL oxidation was induced by Fe2+ and TBARS was measured as the oxidative index . The results illustrated that the inhibition rate (IR)(%) of 2.5, 5, 10, 20, 40mmol/L carnosine on TBARS formation of LDL was 45.3, 54.5,65.7, 76.2, 84.1. In part IV, the linoleic acid oxidation was induced by Fe2+-VitC. the results showed that carnosine was stronger antioxidant preventing lipid peroxidation in linoleic acid systems than that of benzoate. the inhibition rate(IR) (%) of 5,10,15, 20, 25, 30mmol/L carnosine on TBARS formation was16.81,43.81,61.06,73.40, 75.22,82.3. In part V, the reaction of N-Methyl-2-phenylindole with MDA and 4-Hydroxyalkenals was detected in liver homogenates model at 586nm wavelength.. the result showed that inhibition rate of the formation of MDA and MDA+ 4-HNE were53.83% and 54.20% at the concentration of 10mmol/L carnosine. These in vitro observations provide further support for the conclusion that protection against lipid oxidative damage is a major role of carnosine, and the antioxidant activities of carnosine is dependented with its structure.
Keywords/Search Tags:carnosine, antioxidants, TBARS, lipid, peroxidation
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