Effect Of Phenol On The Growth Of Microcystic Aeruginosa And Study On Separation,Physicochemical Properties,Biological Activities Of Acidic Polysaccharides From Microcystic Aeruginosa

This experiment included two parts. In the first part, The effect ofPhenol on the growth of Microcystic aeruginosa were studied under different temperature. The result showed it grows faster under 30C than that of 22 C. Phenol whose concentration was lower than or equal to 200 ug/ml can accelerate its growth while inhibit it when the concentration was higher than 400 ug/ml. After been retreated with Phenol, the alga cell 's photosynthetic O2 evolution rate and soluble protein content decreased, the membrane permeability , the relative content of O2-,MDA increased, the SOD activity decreased and the intensity of its spontaneous fluorescence under treatment was weaker. All these illustrated Microcystic aeruginosa was adapt to summer temperature, and phenol of high concentration could inhibit its growth obviously. The more faster the algaes grow, the more obvious the inhibit effection ..In the second part, Acidic polysaccharide was extracted by boiling water, isolated and purified by DEAE-52 gel column. IR, UV, HPLC and 13C-NMR were used for the structural analysis. The result showed that MAAP was a pure polysaccharide ,the molecular weigh was 2.0028x 105. It's chain was composed of a-glycoside linkage. The component of it was identified as D-Rhamnose , D-Xylose, L-Arabinose , D-Galactose and Galacturonic acid ( the molar ratio : 1.514: 1.457: 1.0: 0.189: 0.583) .The experiment result of Biological activities showed that MAAP can eliminated O2- When its concentration lower than 18 jag/ml. A significant inhibition of tumor growth was observed inthe mice transplanted in the Sarcoma 180(S-180)when they wre treated with MAAP with respect to the control group, MAAP obviously improve the immune function of model mice .protect the thymus, spleen and the normal weight. With the dose of 200 mg/Kg. d , MAAP can improve the SOD activity, GSH content in heart, kidney, brian, liver blood from the treated mice. While their MDA content decrease. All these indicated that MAAP can inhibit the growth of tumor in the body in a certain range.