Study On Flavonoid From Fruit Of Vaccinium Vitis-Idaea L. And Its Biological Activity

Since the implementation of Natural Forest Protection Program, Vaccinium vitis-idaea L. has became one of the economic forest species of recycle economy developing fruit-using industries (drink, brew etc.) with wild resources in forestry system. Meanwhile, the deep development of the fruit residue of Vaccinium vitis-idaea L. (FRV) after primary using will help to prevent secondary pollutant of industry offal, and prolong the industry chain of resource processing.So, this paper firstly analyzed the components in FRV and did qualitative and quantitative analysis of the flavonoid aglycones in FRV by HPLC. Results showed , Two types of flavonoid aglycones, myricetin (0.020%) and quercetin (0.069%), were detected in FRV by HPLC using methanol and water (60:40) as mobile phase at flow rate of 0.8 mL/min. The peak was detected at 368nm. This prompted that FRV had the potential for deep using.On this basis, for realization of deep development of secondary metabolic product in FRV, The optimum conditions of extraction and purification of flavonoids from FRV were studied in this article.Considering the concentration of ethanol, time, temperature and pH as indexes, we studied the extract means of flavonoids, then the optimum conditions of extract was selected by orthogonal design; Adsorption capacity of six macroporous resins (D101, HPD600, AB-8 NAA-9,X-5 and D4020) for total flavonoids were compared. The optimum conditions of adsorbing and purifying total flavonoids from fruits residue with macroporous resin absorbent was selected by orthogonal design. The results showed that ethanol concentration 60%, time 2h, temperature 60℃,w/v 1:20, times 3 were the optimum extract conditions of FFV. The HPD600 resin was best one in the six macroporous resins. The optimum process condition was 60% ethanol as eluting solvent for l0g wet resin/167.5mg total flavonoids, flowing rate lmL/min, absorbing time for lh, the total yield of FFV was 3.89%. The flavonoidaglycones contents of myricetin and quercetin were 6.42% with the characterization analysis of HPLC .In order to identify the physiological activity of FFV on the basis of extraction and purification, this paper continued to study for the effect of FFV on glycometabolism and lipid-metabolism which were not being concerned in the same field of homologous studies.Anti-oxidation of FFV was researched, the results in vitro showed that FFV reduced MDA germination in the liver and kidney spontaneity or induced by the Fe²⁺-Vitc, H₂O₂; restricted erythrocyte hemolysis induced by H₂O₂ or lipids peroxidation in the rat liver; and inhibited oxidation of low density lipoprotein (LDL) induced by Fe²⁺. The results in vivo showed that FFV reduced MDA germination in mouse liver kidney and brain spontaneity, improved the activity of SOD and CAT in liver and serum.The effect of FFV on glycometabolism and lipid-metabolism in normal mouse was investigated. The levels of serum glucose, serum cholesterol(TC) were determined when it lasted 8d,16d,24d and 32d, After 20d glyconeogenesis test was made. The Results showed, FFV reduced the levels of serum cholesterol significantly and serum glucose to some extent, restricted the gluconeogenesis and did not reduce the level of triglyceride(TG) in normal mice.Therefore, alloxan-induce diabetic mice were administered orally with FFV, the results showed that the glucose level of oral administration of 200mg/kg FFV to mouse exhibited significant reduction 18.05% on 7th d, and exhibited reduction 15.04% on 14th(1 and 15.27% on 21thd; 50mg/kg FFV had less reduction and no statistical difference; The effect of control drug was more strong than FFV. 200mg/kgFFV reduced the content of TG and TC, and reduced MDA germination in serum, improved the activity of SOD in liver and serum significantly, and 50mg/kg FFV had not the same effects.Preventive test of alloxan-induce diabetic mice showed that FFV could significantly inhibit the levels of fructosamine and serum glucose and improve the levels of SOD in serum and pancreas.The effect mechanism of FFV on abnormal gl...