Distribution Of Cell Wall Main Loosening Factors-Cellulase And Pectinase In Growing Characean Internodes

The enlargement growth of plant cells is an important aspect during the growth of plant. The hard cell walls have been regarded as a main obstacle for the growth of plant. In recent years the enlargement growth of plant cells were widely accepted as the result of enzymatic hydrolysis of frameworks of polysaccharides in walls, and walls loosen at sub-microscopic levels to make cell enlargement growth happening under the driven by a roughly constant turgor. Some the polysaccharides frame of cell walls, which is believed mainly to be hemicelluloses in between the micro-fibrils adjacent to plasma membrane in about 1/4 of the thickness of the walls, is chopped off and changed the mechanics mold of the walls, and induced loosening of walls.These enzymes are named as cell wall loosening factors. Including the expansin, XET (Xyloglucan Endotransglycosylase), cellulase ,pectinase, and so on. First of all, western blots were employed to prove the existence of cellulase and pectinase in the growing intemodes of Chara corallina. The molecular weight of cellulase and pectinase are checked to be 47KD and 34 KD in growing intemodes of Chara corallina. By observation of confocal laser scanning microscopy on immunoflorecence labeled slices of intemodes of Chara. corallina , we found that at this stage during transport to walls, both of the cellulase and pectinase are co-localized in some organelles that might be the similar vesicles which were found in the observation by immuno-gold labeling around Golgi body in charged of cellulase and pectinase. So data of this experiment show that at transport of cellulase and pectinase toward apoplast are loaded in vesicles from Golgi body.