Cloning, Prokaryotic Expression And Purification Of Functional Domain Coding Gene Of Swine Myostatin

Myostatin, a highly conserved transforming growth factor β(TGF-β) family member that acts as a potent negative regulator of skeletal muscle growth in diverse species. The myostatin gene is expressed almost exclusively in cells of skeletal muscle lineage throughout embryonic development as well as in adult animals . In adult animals, myostatin appears to inhibit the activation and differentiation of satellite cells, resident stem cells in skeletal muscle that are critical to muscle regeneration. Animals lacking myostatin have a dramatic and widespread increase in skeletal muscle mass as a result of a combination of muscle fiber hypertrophy and hyperplasia in mice, cattle, and humans. Similarly, naturally occurring mutations in the myostatin gene also cause the double-muscling phenotype in cattle (2–5). Blocking myostatin activity may have the development of novel muscle enhancing agents for both the therapeutic of human diseases in which increasing muscle growth may be desirable and agricultural applications. From the agricultural prospective, these double-muscling animals may have a dramatic economic returns on the livestock industry since it is projected that they will produce more pork that is of higher quality at a lower cost. However, little is known of the long-term effects on muscle of myostatin blockade, which need a further processed and study. In this study, we express and purify functional domain protein of the swine myostatin, which is to be used as antigen to aquire monoclonal antibody to research the mechanism of swine myostatin and its receptor . First, According to sequence of swine myostatin, a pair of primers with...