Two-dimensional Electrophoresis Analysis Of Arabidopsis Whole-cell Proteins Under Cold-stress

Plants grow under adverse environmental stresses, such asdrought, high salt, or low temperature etc, that affect the growth ofplants and especially the productivity of crops. Low temperature isone of the most common environmental stresses. To study on themolecular mechanism of plant response to low temperature, we usedArabidopsis thaliana Col-0 wild type seedlings as material to explorethe low temperature (4℃) inducible proteins among the whole-cellproteins using 2-DE method. Upon optimization of the wholeprocesses of 2-DE from the preparation of samples through finalproteomics analyses, the whole-cell proteins of stem and leaf fromthree different low temperature treatments, i.e. 24 h, 36 h, 48 h, wereextracted, purified and the differentially induced protein profiles...