| Micro protein pesticide is a kind of new pattern pesticide coming into being lately, which is a group of proteins that play great biological reaction on most plants. Micro protein pesticide may be next wave within pesticide. Either of Harpin protein and Elicitins is a set of protein elicitors which induce HR and SAR of plants. But Harpin protein are separated from plants pathogeny bacterias which has a molecular weight of about 44 KD, while Elicitins mainly derive from Phytophthora and Pythium, which are two kinds of fungies, and their molecular weight are mostly about 10 KD. Neither of Harpin protein and Elicitins is hypersensitive to temperature. The plants can get broad-spectrum resistance to plants pathogeny, which the genes coding Harpin proteins and Elicitins are transformed to. Activator protein is new type protein elictiors differing from Harpin protein and Elicitins by analyzing their sequence of AA(amino acid), and have not induce HR of tobacco. It has a molecular weight from 35-62 KD and is not hypersensitive to temperature and has less identity with the proteins in GenBank. There were not reports about genes coding Activator proteins. Gateway system is a new method of the gene engineering, which mainly includes BXP recombination action and LXR recombination action. cDNA library construction based on Gateway system singnificantly reduces the major drawbacks of conventional cDNA cloning methods, because it eliminated restriction digestion to cDNA. In our experiment, Gateway system was firstly used to construct cDNA library of Alternaria, one kind of epiphytes. As a result, the entry library had a high titer of 1X107 and a total clones of 9X107 cfu, in...
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