Study On Molecular Features Of Small Interference RNA

RNA interference is a new way of specific gene silence,that induced by small duble strand RNA.RNAi is used widely in kinds of research fields , including detection of gene function , diseases therapy ,stem cell, plant resistance to virus and so on.The primary step involved in RNA interference experiment is to screen out the efficient siRNA induceing gene silence from many potential siRNA sequences.That is a work taking time and burdensome.To make the screening work at top speed and feasible, we analysed the efficient siRNA published by Chitest and found that the siRNA has the following features: i A/U at position 1 2 20 and 21; ii G/C at position 3 4 and 6;iiiG/C content of 3'is lower than that of 5'; iv Tm is 31.2℃,with E value[25.1℃,37.3℃].Concerned with others research we supposed these features are tied with the enzymes efficiency involving RNA interference such as Dicer, RISC and RNA depending RNA polymerase.To identify wether these features are available for screening out siRNA,we carried out an experiment that screened out siRNA by following these features.We found that the siRNA could silence the U149 efficeincy.