Effects Of Hyaluronan And Hyaluronan-associated Proteins On The Cumulus Extracellular Matrix In Mice

ObjectiveTo study the effects of hyaluronan(HA) and hyaluronan-associatedproteins(HABPs) on the cumulus extracellular matrix in mice. and to investigatethe effect of HABPs on the the structure and function of the cumulus oophorus.MethodsThree-to four-week-old female mice(wild-type and bikunin-null mice) wereinstimulated to superovulation and isolation of COC, the cumulus oocytescomplexes were counted with microscope. Immunofluoresence staining was used todetect the distribution of HA and HABPs in the cumulus extracellular matrix.ResultsHA and HABPs-positive staining were presented in the cumulus extacellularmatrix. The deficiency of the HABPs impairs the integrity of cumulusextracellular matrix. SHAP and HA were distributed in the cumulus matrix of thewild-type mice. SHAP was not detected in the cumulus matrix of the bikunin-nullmice. HA was distributed between the cumulus cells, but the lattice of the cumuluscells was dismissed, showed that SHAP-HA complex-deficiency cause abnormalityof the cumulus matrix. Immunofluoresence staining indicated HA,LP,CD44 andTSG-6 were presented in the cumulus extacellular matrix. versican was localizedto the expanded cumulus matrix as well as HA, versican expression andlocalization were not altered in the bikunin-knockout mice. SHAP-HAcomplex-deficiency affect the transport of the oocytes. The oocyte countingshows the induced ovulation, there is a significant difference between thebikunin-null mice and the normal controls. In bikunin-null mice, more oocyteswere found in the bursal space between ovary and oviductConclusion SHAP,versican,LP,CD44 and TSG-6 are required for the construction ofintercellular matrices. HABPs is required for the of integrity intercellularmatrices .The function of the oocytes were reduced significantly for themalformation of the matrices.