Physics-Chemistry Abduction On Neurospora Crassa And Analysis By Molecular Ways

Neurospora crassa is a useful kind of fungi belongs to Ascomycetes Sordariomycetida, Sordariales, Sordariaceae, Neurospora. In this experimentation we adopted the ultraviolet ray, microwave, EMS, PYM in this experiment, four kinds of mutagens carried on the Neurospora crassa respectively. And for the mutants, we make them to separate, sieving, keep. And grew with original fungi to carry on the comparison on the appearance structure according to the following standard:1 There is shape of have no grain and grain on the mycelium cell wall.2 The shape of the spore.3 There is shape of have no grain and the grain in the cell wall of the spore and shape of the grain on the spore cell wall.4 The tinct of the spore and the mycelium. The result indicates:1 Many majority changes have taken place in the fungi, can be separated in the configuration with the original fungi. But have the little part of mutant has no bigger variety of occurrence on the appearance, can't be separated in the configuration with the original fungi. For this part can't be distinguish analysed with original fungi, we used the way of molecular biology ( mainly RAPD)to disposal them. The mutant W2, UV1, UV6, UV9, UV10, El, E2, E13and El7 can't be separated in the configuration with the original fungi in the configuration or used the way of molecular biology.2 The best condition of UV mutation is the concentration of C irradiate 10 min, the concentration of D irradiate 5 min. Carried on the research of the first step to the influence of the UV in this text. We detection that use the UV to irradiating the fungi, can also acquire the mutation can be directly separated with the original fungi in the configuration, and for part can't be directly separated with the original fungi in the configuration then can adopt way of molecular biology to separated with the original fungi, but also have some can't be separated with the original fungi, they may be some kind of fungi that have no mutation.3 At the time of disposal with the microwave, we can obtain the better result at the time of short time and long hours, the mutation can be distincted by the microscope, but under the condition of other time, the result is not very obvious, the mutations can't be distinguished through a microscope, need to adopt the way of molecular biology to separated them. To the method, the direct irradiation is easy to make the mycelium has the occurrence change, the accumulation irradiation is easier to make the spore has the occurrence variety. To the spore density, at the time of adopting the direct irradiation, adoption C density is better, but at the time of adopting the accumulation irradiation, two kinds of densities are all can.4 Carry on the processing with the EMS, at 30 minutes—4 hours and 8 hours—48 hours can get the better result, the mutation can be separated through microscope. See from the density of EMS, the medium and low density(X, Y)can get the better result. From the spore density, the density of D can be better.5 Carry on the processing with the PYM,30 minutes—2 hours,6 hours—24 hours can get the better result. From the density of mutagen, the low density (a) can get the better result. From the spore density, low density of mutagen adopt the low spore density, but high density of mutagen adopt the low spore density can get the better result.6 From the comprehensive on trial result, the result of PYM is better than other three kinds of mutagen.