Translocation Of Single Polymer Chain From A Nanopore On A Membrane

Translocation of polymer chain through some special channels on the cellular membrane has considerable important significance in biological field. Recently, the experimental techniques based on this interesting phenomenon have been used in the direct, high speed detection of sequences in single polynucleotide molecules. In addition, the translocation process also attracts significant attentions from many researchers for it refers to many basic problems in the field of soft matter physics. In this dissertation, we summarize the current experimental and theoretical progresses, and analyze the results proposed by a few groups, and based on which we also make some investigations on this translocation problem.The dissertation of M.S. degree is mainly composed three chapters:The first chapter: Summarize. We carry on a simply review of the translocation of the single polymer chain investigations in recent years, and give a detail summarize of the experimental and theoretical progresses respectively.The second chapter: Theoretical investigations on the single polymer chain translocation. In this chapter, as an extension of the former works, we investigate some interactions effect on the single polymer chain translocation. According to the polymer physics and biophysics knowledge, we know that some special interactions would induce the configuration of the polymer chain changing, and which therefore lead the free energy changing correspondingly. Based on that, we consider that there are three kinds of interactions mainly affect the configuration of the polymer chain, which are the chain-solvent interaction(solvent effect), the chain-chain interaction and the interactions between the side groups of the polymer chain. Then we calculated the mean first passage time(MFPT) under different conditions.The last chapter: Prospect. Anticipating the theoretical investigation direction of the single polymer chain translocation, and providing several possible theoretical models for our continue investigations.