Construction And Expression Of Hepatitis B Virus Genome Eukaryotic Expression Vector Carrying Enhanced Green Fluorescence Protein

Hepatitis B virus (HBV) is a member of the Hepadnaviridae family. One characteristic property of HBV is their high species and tissue specificity. HBV researches is always based on cell culture and small animal models. The transgenic mice harboring HBV complete genomic DNA consistently supports virus gene expression and replication, specially in serum and liver, It is unsafe to breed and use HBV transgenic mice in normal laboratory. It always takes time to estimate HBV transgenic mice by conventional technology arduously. HBV transgenic mice with the enhanced green fluorescence protein expression in all tissues should be practicable and effective in breeding and estimation. In this study, we have constructed pCX EGFP-HBV adr 2.0 eukaryotic expression vector harboring 2 copies of HBV genomic DNA and EGFP gene by recombination DNA technique. The results of L02 transfection and hydrodynamic injection suggested that pCX EGFP-HBV adr 2.0 could express in vitro and in vivo. 10kb DNA fragments of pCX EGFP-HBV adr 2.0 were usded to generate HBV transgenic mice with EGFP reporter gene.