1.Regulation Of SGK By Corticotropin-Releasing Hormone In Hippocampal Neurons And Its Mechanisms 2.Effect Of Glucocorticoid Exposure In Late Gestation On Expression Of CRHR And SGK In Offspring's Hippocampus

Abstract: Corticortropin-releasing hormone (CRH) is an important hormone in neuroendocrine system, which plays a wide role in central nervous system and its mechanisms are complicated. CRH can bind to its receptors, activate different G proteins, and participate in physiological functions of hippocampus. Serum- and glucocorticoid-regulated protein kinase (SGK) is regulated by many steroid hormones. In order to study the regulation of SGK by polypeptide hormone CRH and its potential physiological significance, first part of this study observed regulation of SGK mRNA and protein expression by CRH in cultured rat hippocampal neurons and relationship between this regulation and neuron survival. We found CRH binding to CRHRl induced SGK expression via PKA pathways. Previous studies shows glucocorticoid (GC) in gestation has an imprinting effect on CRH neurons of hippocampus. Therefore the second part of this study observed effect of GC overexposure in late gestation on SGK regulation by CRH in offspring's hippocampus. Our results showed GC receptors, CRH receptors and SGK genes expression were all decreased in offspring's hippocampus after birth and this decreasing continued at least for seven days.