| Cellulose is by far the most abundant carbohydrate available on earth, and can be converted into glucose by cellulases.Cellulases have been attracted increasing attention due to their application potential in the field such as bioconversions of cellulose waste, textile industry ,paper and pulp industries, deetergent industry protoplasts generation from yeast and filametous fungi and so on. However, there have several limits ,for example, degradation ,high cost and environment pollution, on hydrolysis cellulose by chemical methods. In contrast, biodegradation process not only increase utilization ratio but also gain greater benefit.Cellulases from the themophilic fungi have reported to be stable at high temperature and like mescophilic fungi, the thermophilic fungi ,producemultiple forms of the cellalase components.Chaetomium thermophilum CT2, one of the thermophilic fungi, is a metatrophic soil fungus,and can be thrive at temperature between 45 and 50℃,while most fungi will die above 40℃. C.thermophilum CT2 is shown to produce themostable enzymes such as protease, glucomyces, lipoxygenase, cellulases. Endoglucanse from the fungi have been studied. In the present study, a new extracellular endoglucanase from C. thermophilum CT2 was produced when it was grown in minimal liquid medium containing lactose as the only carbon sourse. The enzyme was purified from the culture filtrate of the strain by fractional ammonium sulphate precipitation, Phenyl-Sepharose chromatography and DEAE-Sepharose chromatography. The purified enzyme was shown to be homogeneous by SDS-PAGE, and the molecular weight was estimated to be 30kDa. Then characterize of the purified enzyme was carried out.
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