The Light Information Analysis And Application On Biomacromoleculers And Environmental Biomicroparticles

In this contribution, we characterized and analyzed biomacromolecules and biomicroparticles, based on the common absorbance, fluorescence and light scattering method. We have analyzed the mechanism and essence of the spectra phenomenon, and obtained the useful information which can provide some theory and experimental basis for their application on clinical diagnosis, biochemistry, environment science etc.Both scattered light signals and the fluorescence signals were twins in fluorospectroscopy, and the light scattering signals are always the interference sources of spectrofluorometry. In order to investigate the relationship between the light scattering and fluorescence in the spectrofluorometric measurements, we have observed that the signals by RLS mode contain fluorescence signals for some species, particularly for organic small molecules (OSMs) with small Stokes shift or excitation and emission spectra overlapped in our experiments. Considering that OSMs are very important acting as binding guest to probe the biomacromolecules and environmental acceptors, herein we take the azo dye—Lignin Pink (LP) as an example and try to discuss the light scattering and fluorescence emission signals of OSMs by synchronous scanning the excitation and emission monochromators of the spectrofluorometer. Investigations showed that LP had four emission peaks which were characterized by different properties. The light emission at 282.0 nm and 344.0 nm could be attributed to the resonance light scattering (RLS) signals and that at 420.0 nm and 570.0 nm are composed of both RLS and fluorescence emissions, respectively. UV-Vis measurements showed that LP doesn't have the tendency of aggregation, and that the strong RLS signals should be ascribed to the large hydrodynamic diameter of LP itself in aqueous medium, supported by dynamic light scattering (DLS)...