Phylogenetic Analysis Of The Relationship Between Hystrix And Leymus And Pseudoroegneria Its Affinitive Genera (Poaceae: Triticeae) By Using Genes Encoding Plastid Acetyl-CoA Carboxylase And EST

18 sequences of gene enconding plastid acetyl-CoA carboxylase were obtained. The phylogenetic relationships between Hystrix species and its affinitive species was analyzed. The results were shown: (1) all species have only one cope of the Acc-1 gene except Hystrix patula. There are two copies in Hystrix patula, named H. patula plal and H. patula pla2; (2) the Hy. patula plal and Pseudoroegneria spicata (St) was sister group with a bootstrap value of 87%, while the Hy. patula pla2 and Elymus wawawaiensis (StH) was sister group with well bootstrap support, and this sister group formed a clade with Hordeum L. This results provided a molecular evidence for Hy. patula containing StH genome; (3) the sister group was not in beween Hy. patula and the other three diploid of Pseudoroegneria but Hy. patula and P. spicata. There is not subclade in the other three diploid of Pseudoroegneria. This results indicate that P. spicata is one of the progenitor of Hy. patula in north America; (4) the clade of Ns genome including Hystrix Moench three spcies and all the spcies in Psathyrostachys and Leymus. Hy. duthiei ssp. longearistata and Hy. duthiei ssp. duthieiwas a sister group with a bootstrap value beyond 87%, which indicated that they have close affinity. In addition, the two copies of Acc-1 genes were discussed in the present paper.Plastid acetyl-CoA carboxylase catalyzes (ACCase) the first step in de novo fatty acid biosynthesis. Gene encoding plastid ACCase (Acc-1) have been used to study systematics and evolution of annual species in the tribe Triticeae. However, related study have not been reported in perennial species of Triticeae. In this study, the results are shown that the size of the eighth exon and the seventh intron of Acc-1 gene in Elymus wawawaiensis is more 176 bp than that of previously published excluding nucleic acid substitute by using sequence comparison, and there is a remarkable difference between this sequence and its related sequence which published. The highest comparability 87.15% was shown when this sequence have been compared with Hordeum vulgare Acc-1. The Acc-1 genes of Panicum virgatum, Zea mays and Lolium rigidum were regarded as outgroup. A phylogenetic tree was established by maximum parsimony and neighbor-joining method respectively. Elymus wawawaiensis and Hordeum vulgare formed a clade, the bootstrap value is 100%. At the same time, on the study of systematics and evolution of perennial species in the tribe Triticeae, the Acc-1 gene have important perspective of application as the present paper shown.Phylogenetic relationships of diploid species of Pseudoroegneria (Nevski) A. L(o|¨)ve...