Genotyping Of Alcohol Dehydrogenase-1B And Aldehyde Dehydrogenase-2 By Using Denaturing High-performance Liquid Chromatography

Background and ObjectivesHuman alcohol dehydrogenase-1B (ADH1B) and aldehyde dehydrogenase-2 (ALDH2) are among the most important enzymes in the complex process of alcohol metabolism. ADH1B is situated in 4q22 and ALDH2 is situated in 12q24. The polymorphic variants G143A in exon 3 of the ADH1B gene and G1510A in exon 12 of the ALDH2 gene, which encode enzymes with altered kinetic properties, are the most well known and are related to the development of alcohol dependence, particularly in populations of Asian origin. Recently, genetic variants of alcohol metabolism, including ADH1B and ALDH2 polymorphisms, can partially explain susceptibility to some human disorders, such as human cancers, liver dysfunction, coronary heart disease, diabetes complications and polyneuropathy. Therefore, simultaneous genotyping of these two polymorphic loci would be useful for both estimating the risk of alcohol-related diseases and investigating the association between these enzyme polymorphisms and other alcohol-related problems.Recently, a range of molecular approaches has been used for allele discrimination of polymorphisms in the ADH1B and/or ALDH2 genes, such as RFLP, PCR-CTPP, SSCP, APLP, MAMA. Each of these approaches has advantages and limitations, but...