The Study Of Skatole On Some Properties Of Important Digestive Enzymes

Skatole ( 3-methylindole ) which has strong odour is metabolic product of tryptophan in human and animals. It also exists in many food we take ( e.g some meat). Skatole is harmful to human animals as well as environment. When human and animals take food which contains skatole or can be converted into skatole, skatole will inevitably interact with digestive enzymes. Skatole absorbed through skin may also interact with digestive enzymes in human and animals. So we studied the effect of skatole on three important digestive enzymes.We chose a-amylase which is important in digestion of amylum and saccharide as well as pepsin and trypsin that play key role in digestion of proteins. First we studied the effect of skatole on activity of a-amylase pepsin and trypsin, then we studied the effect of skatole on structure of three important digestive enzymes by UV spectra and fluorescence spectra.It was found that skatole could inhibit those three digestive enzymes. The inhibition ratio of skatole on trypsin was highest, whereas that of pepsin was lowest. When the concentration ratio of skatole to enzymes is 5/1, the inhibition ratios of skatole on those three enzymes are 32.4 % 28.7% and 26.6%.When adding different concentrations of skatole into those three enzymes, the...