Study On Monitoring Electrophile Chemical Pollutant Based On Transgenic Cell

Electrophile chemical is a large class of environmental pollutants which can combine with nucleophilic center of large molecules directly in cell biology or bind the center by bio-transformation to produce biological compounds. This chemical can not only cause acute and chronic poisoning, but also cause cancer. Electrophile chemical is the major pollutant in the environment, this is very concerned by many of environmental scientists.Most of environmental pollutants are electrophile chemicals, such as TCDD,CdCl₂, HgCl₂,Pb(C₂H₃O₂)₂ and so on. Many foreign researchers have found out that some special motifs in the genes of Mammals cells, which can specifically respond to electrophile chemicals. This motif is called Electrophile Response Element(EPRE). The region of the motif is very conservative, which regulates the expresssion of downstream reporter gene. The antioxidant genes such as r-glutamylcysteine synthetase and cysteineglutathione transferase gene are mediated by EPRE. The degrees of the gene expression have some relationships with EPRE activation degrees caused by electrophile chemicals. The dose-effect model exists between them.TK(thymidine kinase) and MT(metallothionein) minimal promoter was selected from the plasmid of p(Gal4)5-TK/CAT and pMT/v5-his according to the pricinple of mammalian cell two hybrid.The two promoters were ligated with luciferase reporter gene respectively,and then the artificial electrophile response element (EPRE ) were inserted into the upstream of luciferase reporter gene.In this way,The luciferase reporter...