Research Of Mouse Early Embryos Culture In Vitro

The technique of early embryos culture in vitro is an important tache to the mammal embryos engineering technology. No matter in research field, or in production field, it plays an important role. Many scholars have made lots of excellent researches to perfect this technique. But up to today, the technique is far from perfection because of the low blastocyst production rate and the poor quality of the blastocyst. Most of the researchers paid more attention to the improvements of the culture media. They think that the method is perfect, which is used to culture the embryos. But, actually, the environment is dynamic and supplied by the oviduct epithelia, in which the early embryos developed. On the purpose of indicating whether this dynamic physical environment can induce the development of the embryos. We made a fluid culture device for model the dynamic environment of embryos culture in vivo. And we used fluid culture device, open culture method and normal micro-drop culture method to culture 2-cell mouse embryos. Then we made the compare of the culture result.Experiment 1: To choose the fit flow rate:This experiment was made to choose a suitable flow rate for the flow culture system. Three groups of different flow rate were used to culture 2-cell mouse embryos, 0.05ml/h; 0.1ml/h and 0.2ml/h. Then we compared the blastocyst rate of each group after 72h culture in vitro. The result shows that the group at 0.05ml/h flow rate had make more blastocyst(73.3%, 22/30). The blastocyst rate of the group of 0.1ml/h and the group of 0.2ml/h are (51.6%,15.5/30) and (35%,10.5/30). The differences of the blastocyst rate of the three group are bally markedness(p<0.01)...