The Roles Of Hsp90 Under Oxidative Stress And Adaption In HepG2 Cells

BackgroudThe environment affects all living organisms. An organism must adapt different changes if it is to survive. Environmental changes, irrespective of source, cause a variety of "stresses" or "shocks" that a cell must face repeatedly, and to which its genome must respond in a programmed manner for the organism to survive. Examples are responses to light, oxidative stress, pathogenicity, wounding, anaerobiosis, thermal shock. In the course of evolution, organisms evolved a complex array of mechanisms for adapting to both minor and major fluctuations in the environment. Oxidative stress is a kind of stress injuries, it can induce celluar death or physiological functions changes by ROS. The latter change can be mainly defined adaptation or tolerance to stress following a sublethal stress experience. Hsp90 is a member of Molecular chaperon and can protect cells from stress injury. Hsp90 can interact with many signal transduction pathway in cells.Here we studied the roles of Hsp90 in HepG2. How Hsp90 protects the cells in oxidative stress and adaption must be solved. It's a key question between the enviroment and living organisms. ObjectivesObserve the expressions of Hsp90 in HepG2 under oxidative stress and preconditioning, and study the mechanism of its cellular protection. Methods1. Establish preconditong and oxidative stress model of human cell line HepG2. HepG2 cells were seeded onto 24-well plates,96-well plates and culture bottles...