Study On Introducing FMDV Epitopes Fused Gene Into Carrot Mediated By Agrobacterium

Foot-and-mouth disease (FMD) is the world-wide infectious disease induced by Foot-and-mouth disease virus (FMDV). It mainly infects the even-feet domestic animals. And its character is such as more broadly infectious approach, easy infection, high disease-deadly percentage. Vaccines derived by transgenic plants is becoming one focus of plant genetic engineering. And that has such advantages as easy production, low cost, convenient and safe use and easy storage. Carrot plants are broadly growed in china. And its character is abundant nutrition, uncooked eating. So it is considered as an ideal plant for studies of transgenic plants vaccine.The studies mainly presented that Fused gene O21-O14-A21-HBcAg containing both type O and A of FMDV epitopes were introduced into carrot callus mediated by Agrobacterium. Hygromycin-resistant carrot callus were obtained by the medium containing antibiotic.Hygromycin-resistant carrot plants were regenerated from the resistant callus. The correlative detection experiments were finished, such as, GUS expression detection in transformed callus and Hygromycin-resistant carrot plants; PCR, PCR-Southern blotting and RT-PCR for Hygromycin-resistant carrot plants.The mainly important experiment results were as follows:1. Carrot regeneration system was established. Culture medium for inducing carrot callus was MS + 2,4-D 0.1 mg/L, culture medium for carrot plants regeneration was MS medium without any phytohormone and culture medium for rootage was 1/2MS...