Cloning, Prokaryotic Expression And Multi-tissue Expression Of Heat Shock Cognate 70 Gene From Litopenaeus Vannamei

Heat shock proteins (HSPs), also known as stress proteins, belong to one of highly conserved protein families. It is widly found in most cells or organs, from bacteria, yeasts, plants and animls. Under normal conditions, certain HSPs constitutively express in cells, and the expression of those inducible HSPs can be induced by a number of physiological signals, such as anoxia, energy depletion, injury, hyperthermia, heavy metals and pathogens. Eukaryotic HSPs are composed of HSP105/HSP100, HSP90, HSP70, HSP60 and low-molecular weight HSPs. The heat-depandent HSP70s is one of most representative families and undertakes fundamental functions in cells. Therefore, HSP70s have intrigued researchers' great interest in its actions and mechanisms. To date, we know that HSP70s play an essential role in protein metabolism in both stressed and unstressed cells. HSP70s are implicated in nascent protein folding, membrane translocation, formation and degradation of denatured proteins. In addition, HSP70s demonstrate its potential protection against environmental stressors and participate in immune intermediation. Because of speciality of HSP70s, further studies on interaction between HSP70s and environmental adaptability in aquatic animals would greatly ameliorate aquatic animals' distribution, survival and reproduction.We determined the sublethal and lethal temperatures for Litopenaeous vannamei via heat-shocked experiments. L. vannamei was treated by a jumped temperature (from 25 ℃ to 35 ℃) for 2 hours and then exposed to the lethal temperature (40 ℃) water, the survival rate increased up to 54.2 %. After exposed to the sublethal temperature for 2 hours, four divided groups of L. vannamei were maintained at room temperature (25±0.5℃) for 2, 6, 12 and 24 hours, respectively. Subsequently, the shrimps were injected with white spot syndrome virus (WSSV). The result showed that the mean survival time of the infected shrimps with recovery times of 2, 6,12 and 24 hours was 101.43,98.07,76.92 and 63.28 hours, respectively. The mean survival time of the control group, which was directly injected with WSSV without treatment of sublethal temperature, was 46.89 hours. The mean survival times of the...