Clone, Expression, Purification And Crastallization Screen Of Two Genes From Shigella Flexneri2a301 And The Analysis Of Sf2088 Aggregation

Shigella species are Grim-negative, non-sporulating, facultative anaerobes causing bacillary dysentery or shigellosis in man with estimated annual episodes of 160 million and 1.1 million deaths. In China, more than 10 million cases are estimated per annum, of which 50-70% are caused by Shigella flexneri serotype 2a and most are associated with epidemic and pandemic shigellosis. Shigella are highly invasive in the colon and the rectum, and are able to proliferate in the host cell cytoplasm, triggering an in ammatory reaction. The clinical manifestations of Shigella infection vary from short-lasting watery diarrhea to acute in ammatory bowel disease characterized by fever, intestinal cramp and bloody diarrhea with mucopumlent feces. Since the current eventive and treatment strategies are found to be inadequate, the World Health Organization has placed an anti-Shigella vaccine as a priority.