The Morphologic And Immunohistochemical Studies On Alimentary Canal Of Wolves And Domestic Dogs

In This Study, some morphologic indexes alimentary canals of wolves (Canis lupus) and domestic dogs (Canis familiars) were measured and made comparison, and identification, distribution and morphology of endocrine cells in the digestive tract of wolves and domestic dogs were studied by using immunohistochemical techniques with seven gut hormone antiserum. It is similar in anatomy and histology in the configuration of digestive tracts of wolves and domestic dogs in the large. There is no remarkable difference in the cubage of the stomach of wolves and domestic dogs. Wolves have lower total length and also lower total weight of digestive tract than domestic dogs, and these differences are significant. These differences are caused generally by the difference of large intestine of these two kinds of animals. Though the comparison of the thickness of each sect and each layer of the digestive tract of these two kinds of animals, it can be found that, wolves have thinner oesophagus and cardiac mucosal fold but thicker large intestine mucosal fold. By the immunohistochemical study of endocrine cells, it indicated that, 5-HT-IR cells and Gastrin cells were extensively distributed. Vasoactive intestinal polypeptide cells, Somatostatine cells, Substance P cells and Glucagon cells were primarily distributed in the gastric mucosa. It could also be found in some places of small intestines and large intestines. There was less Pancreatic polypeptide cells in the guts of wolves and domestic dogs. The comparison of morphologic of alimentary canal and the distribution of endocrine cells indicated that the structure of digestive tract of wolves and domestic dogs were generally the same.