The Permeable Mechanism And Research Of Cell Membrane Under Pulsed Electrical Field

The fundamental effects of an electric field on a cell can be described by considering the cell to be a conductive body (the cytoplasm) surrounded by a dielectric layer (the cell membrane).When a short electric pulse is applied to this cell, the resulting current causes accumulation of electrical charges at the cell membrane and consequently a voltage across the membrane. If the membrane voltage exceeds a critical value, structural changes in the surface membrane occur with transmembrane pore formation, a process known as electroporation. The applications and mechanistic understanding have received growing attention over the past two decades.Several classes of applications have been identified, with in vitro electroporation widely used to transfect suspended or anchored cells in laboratory apparatus by introducing DNA. Essentially, any size molecule can be introduced into the cell cytoplasm or transported across a membrane. In vivo applications include the local delivery of potent but relatively membrane impermeable anticancer drugs into solid tumors. Electrochemotherapy (ECT) has now reached clinical trials. Motivating in vivo experiments showed that orders of magnitude more anticancer drugs are delivered into cytoplasm than by passive permeation. New anticancer treatment, which consisted of established drugs with added electric pulse, would in principle be attractive.Firstly, the thesis introduces the research on the mechanism and development of electroporation and its applications.Secondly, it introduces the gene pulser transfection apparatus which was designed by our group. We make a point of the principle and specifications of this apparatus. It includes the high-voltage generator, thyristor trigger circuit, single processing circuit and capacitance of combination. Because of the temperature influence, we must examine the temperature in the experiment. We propose optical fiber sensor in the apparatus.Thirdly, theoretic model of the electroporation is analyzed. With the research on the mathematic and physical model, the research is referenced by the experiment. And the relationship between the effect of electroporation and the electric parameter is introduced.At last, we reported a new approach for the treatment of sarcoma, Electroporation Therapy (EPT). Based on the experiment of Sichuan University, we improved the method of the electroporation and the experiment got good results. At the same time, the medical expert recognized the result. From the experiment, we discovered the approach could obtain remarkable effect.