The Research On The Interaction Of DNA With Bioactive Molecules

DNA is the carrier of genetic information and plays an especially important role in the course of genetic. Investigation of DNA is important in the life sciences field. DNA is the main target molecule that exists in anticancer and antiviral therapies. All cell and virus carry DNA. Normal heredity function of DNA depends on the physical and chemical interaction of other molecules with DNA.Interaction of DNA with small molecules, affected physiological and physical chemical character of DNA and changed the transfer and copy of DNA. Hence it is meaning that interactions of small molecules with DNA are investigated to understand bind of DNA with protein, anticancer mechanism and to design and synthesize new drug. In medicament, it possible to foresee major steps forward in our understanding of the molecular basis of disease, both from attack by external pathogens and internally from variations within the human genome resulting in a plethora of new molecular therapeutic targets for drug design and discovery.A variety of techniques from molecular biology have been used in research laboratories to study the effects of DNA binding small molecules, such as the gel mobility shift assay, ¹H NMR, electrochemistry analysis, DNA foot-printing assay, and fluorescence-based assays, UV-Vis spectrum and viscometry.The first Part: General Introduction and ConclusionIn this part, the development in the studies of DNA, including sturcture of DNA, interaction of DNA with other matter and the mothods about interaction of DNA and small molecule are reviewd. The important theoretical and practical significance of the research of the interaction of DNA and bioactive molecules in biochemistry are discussed.The second Part: Electrochemical studies of the interaction of quercetin with DNAIn this part we studied the electrochemical behavior of quercetin interaction with DNA by SECM and EIS. The interaction has different electrochemical information. Quercetin can intercalate DNA and form a kind of electrochemical inactive supramolecular complex. The charge transfer rate constant for the K₃Fe(CN)₆/K₄Fe(CN)₆ couple was obtained. The results provide new insight into rational drugs design and would lead us to further understanding the information of interaction between anticancer drugs and DNA.The third Part: Electrochemical, spectral and viscometric studies of the interaction of brilliant crystal blue with DNAFrom results presented in this part, we confirm that in buffer solution BCB undergoes two single electron redox processes involving two protons resulting in redox of 3-amidogen and 6- nitrogen. Also, the results from the various experiments show that BCB can bind with DNA by electrostatic mode. In addition, the apparent rate constant of the redox active probe in solution were obtained simultaneously. The results provide new insight into rational dyes design and would lead us to further understanding of the interaction mechanism between dyes and DNA and application in the chemistry, iatrology and biology kingdom.