The Study Of The Function Of The Promotor In Arobidopsis IAA2 Gene And Its Subcellular Location

Auxin biology is one of the oldest fields of experimental plant research. Auxin play a critical role in numerous aspects of plant growth and development on molecular, cellular and the whole level. Although auxin was the first plant hormone identified, insights into the molecular mechanism of auxin action is only recently beginning to emerge. Two recent Nature articles reveal that the F-box protein TRANSPORT INHIBITOR RESPONSE1 (TIR1) is a receptor for auxin. Auxin signaling in plants includes five critical factors------the auxin receptor, SCFTIR1 complexes, Aux/IAA proteins, ARF, and AuxRE. Aux/IAA proteins are active repressors. Mutations of Aux/IAA genes lead to the abnormal phenotypes. Aux/IAA genes were first isolated as members of a family of genes that were rapidly induce in response to auxin (indole-3-acetic acid, IAA). The Arabidopsis genome has 29 genes that encode putative Aux/IAA proteins. The Aux/IAA function is poorly understood. Promotor of IAA2 gene was fused to reporter gene to reveal their expression patterns in transgenic plants. Coding sequences of IAA2 was fused with EGFP to indicate their subcellular locations. Results as bellows: Promoter of IAA2 gene expressed in microtubules, and was induced by auxin rapidly. IAA2 protein was located in nuclear. The work in this thesis will be beneficial for studying the function of IAA2 gene.