β-Xylanase From Aspergillus Niger SL2-111: Purification, Characterization And Gene Isolation

Xylanases have important application in animal feed industry, paper making and others.In this subject we had purificated two xylanases from Aspergillus niger SL2-111, and the gene sequence of one xylanases from Aspergillus niger SL2-111 had been isolationed.1 Purification and property of xylanases from Aspergillus niger SL2-111We use Aspergillus niger SL2-111 to ferment the substrate.The (NH₄)₂SO₄ was added to the culture filtrate, and the precipitated material was collected by centrifugation. Then desalted on Sephadex G-25. The xylanaseâ… was purified by DEAE-Sepharose Fast Flow and Sephacryl S-100 High Resolution.The xylanaseâ…¡was purified by QAE cellulose anion exchanger and Sephacryl S-100 High Resolution. The property had been researched.2 Gene-clone of xylanase from Aspergillus niger SL2-111We got total RNA from Aspergillus niger SL2-111. Then it was synthesized to be first-strand cDNA. The full-length gene encoding one xylanase was obtained by PCR amplification.