Cloning And In Situ Detection Of DSPPs And Enamelins In Tooth Germ

The tooth development is similar to other organs,which is also dependent on thereciprocal induction between the presumptive tooth-forming epithelium and the overlyingmesenchyme,Tooth germs progress through a well-characterised path of sequential andinteraction, in which the epithelium differentiates into ameloblasts,and theectomesenchyme gives rise to the odontoblasts, and then secrete matrix proteinrespectively to form enamel and dentin finally.enamel and dentin can be used for the morphology index of tooth differentiation,which mark the morphological evidence of tooth development.And the differentiation ofameloblasts and odontoblasts can be used to detect the tooth differentiation by cellbiology level.The cell differentiation can base on the tissue morphology construction ofcells,however, detecting the cell differentiation by histology and morphology level is farfrom enough,so we must establish detection method from molecular level..In this research,both human and mouse DSPP and Enamelin cDNA fragements are cloned from the exonof the genomic DNA. DIG labeled antisense mRNA probes are prepared by in situhybridization of DSPP expression in odontoblasts and Enamelin expression inameloblasts of human and mouse. Our results show that the probes can specificallydectect DSPP expression in odontoblasts and Enamelin expression in ameloblast of eitherhuman or mouse respectively and can be used as a molecular tool for examing thedifferentiation of odontoblasts and ameloblasts in human and mouse.