Organically Modified Silica Nanoparticles Mediates Survivin Gene Delivery To Olfactory Ensheathing Cells

Objective: To study Survivin gene modified olfactory ensheathing cells (OECs) with the Organically modified silica (ORMOSIL) nanoparticles.Methods: After established ORMA40 nanoparticles, transmission electron microscopy was used to determine the morphology and the size of the aqueous dispersion of nanoparticles. Examination of Survivin plasmid and the complex formation of Survivin plasmid with ORMA40 nanoparticles. The olfactory ensheathing cells (OECs) of SD mouse were isolated and purified in vitro. And the olfactory ensheathing cells (OECs) were evaluated by the immunocytochemical expression of anti-rabbit p75. The Survivin plasmid was used for transfection in olfactory ensheathing cells (OECs) with ORMA40- Survivin complex suspension. Fluorescencemicroscope observed expression of enhanced green luorescene protein (EGFP). Three days later, we make an evaluation on the expression of Survivin by immunocytochemical stain.Results: Transmission electron microscopy images showed that the particles are highly monodispersed in the aqueous dispersion of nanoparticles ORMOSI.. After treatment with DNase 1, the free plasmid was completely digested, whereas the Survivin plasmids bound to the ORMA40 nanoparticles were completely protected. It was proved that olfactory ensheathing cells (OECs) could be purified by the immunocytochemical expression of anti-rabbit p75. The transfected olfactory ensheathing cells (OECs) with ORMA40-mSur complex suspension express Survivin and enhanced green luorescene protein(EGFP). The transfected olfactory ensheathing cells (OECs) with ORMA40-pSur complex suspension express Survivin.Conclusions: The results show that the nanoparticles ORMA40 mediate Survivin plasmids transfecting olfactory ensheathing cells (OECs) is simple and efficient, and is an ideal method for gene transfection.