Application Of Microfluidic Chip On Organic Reactions And DNA Separation

Miniaturized Total Analysis Systems (TAS) or called Laboratory on a Chip(LOC) is a distinct new field which has the potential to influence many subject areas. Microfabricated fludic devices are powerful tools for performing chemical and biological assays with increased speed, relibility and reduced sample consumption and cost. Forthermore, microfluidic reactors have a number of advantages over conventional chemical laboratory batch methodologies, such as short molecular diffusion distance, large specific surface area, high mass and heat transfer capacity, which would be expected to promote highly effective chemical reactions in the microfluidic reactor. The aim of this paper is to explore its application area and resolve some problems related to its extension to commercial use.In the chapter 1, the main features and advantages of microfluidic reactors, the method to drive liquid through the microchannels, and the status of CE separation DNA fragments by using microfludic chips were summarized.In the chapter 2, the reaction for synthesis of 2-ethyl-2-phenyl-3-bromo-3-butenal from 1-phenyl -2-ethyl-2,3- butadienol and Br₂ was carried out in glass microchannel reactor. The effect of flow rate and reaction time on yield carried in microfluidic chip was determined. The reaction has been found to be more efficient than that in a round-bottomed flask with vigorous string. The demonstrated advantages of organic synthesis in microfluidic chip include faster reaction rate, less consumption of reactants and labor contaminant, which proved microfluidic chip to be a powerful tool for synthetic application.In the chapter 3, the chip-based electrophoresis was used for the separation ofφⅩ-174/Haeâ…¢DNA restriction fragments on coated glass microfluidic chip with the low-viscosity sieving matrix HEC. The concentration of sieving matrix and its use-period were optimized. The effect of preservation condition of coated microchip on the separation efficiency of DNA fragments was evaluated. A method for conserving coated microchip was suggested to prolong its longevity.