The Analysis Of The Key Enzymes Of Arginine Biosynthesis For Corynebacterium Crenatum

L-arginine is widely applied in the fields of food and medicine as an importantamino acid for the human body's metabolism. In this article, The function of argR inarginine biosynthesis was studied through analyzing the relation between the capacityof producing arginine and the fermentiation conditions among the mutant named theC. crenatum A. S. M2, and two engineering strains named C. crenatum A.S.M2â–³argRand C. crenatum A.S.M2.sp. The key enzymes of arginine biosynthesis wereinvestigated by the method of affinity chromatography, SDS-PAGE and the MatrixAssisted Laser Desorption Ionization time of flight mass spectrometry(MALDI-TOF-MS). The main results are illustrated as following:(1)The growth curve and the utility rate of carbon, the pruducing curve ofarginine and the pH of the culture medium of C.crenatum were determined. Theexperimental result shows that C.crenatum A.S.M2.sp is the bacterium which hasthe highest yield of arginine. The yield of this bacterium gets to 85mg/mL. Inconclusion, argR gene of C.crenatum is a positive regulator in the argininebiosynthesis.(2) The bacterium was gathered in the highest yield period of producing arginine.The water soluble proteins of these bacteriums were extracted and the specialenzymes and proteins were separated by the methods of affinity chromatography..(3) The special enzymes and proteins with an affinity for the arginine wereanalyzed by SDS-PAGE and 10 protein dots were pick out and then to go throughpeptide mass fingerprinting based on MALDI-TOF-MS and NCBI databasesearching.